ANTIBACTERIAL AND ANTIOXIDANT POTENTIAL OF ETHANOL EXTRACT OF TECOMA STANS (L.) JUSS. EX KUNTH LEAVES
DOI:
https://doi.org/10.36103/h3e89y10Keywords:
alkaloids, bignoniaceae, free radicalsAbstract
The aim of this research is to evaluate the total phenolic content, antibacterial activity and antioxidant activity of Tecoma stans. leaves were collected from the College of Veterinary Medicine, University of Baghdad, and the ethanolic extract was prepared using a magnetic stirring. Phytochemical screening revealed the presence of bioactive compounds such as flavonoids, phenols, alkaloids, tannins, saponins, and glycosides. Antioxidant activity was evaluated using the DPPH assay, along with Total Phenolic Content (TPC) analysis. The extract exhibited strong antioxidant activity that increased with concentration. High-Performance Liquid Chromatography (HPLC) analysis identified six major phenolic compounds: quercetin, gallic acid, caffeic acid, apigenin, ferulic acid, and rutin. The extract also demonstrated antibacterial activity against multidrug-resistant Pseudomonas aeruginosa at concentrations of 128 and 256 mg/ml, with inhibition zones ranging from (12.33 ± 0.42) mm to (21.67 ± 0.42) mm, respectively, indicating its potential as a promising natural antibacterial agent.
Received: 22/3/2025
Accepted: 25/6/2025
Published: 30/9/2026
References
Al-Khafaji, A. R. & H. A. Al-Azawi. 2022. Green method synthesis of silver nanoparticles using leaves extracts of rosmarinus officinalis. Iraqi Journal of Biotechnology, 21 (2): 251-267.
Al-Quhli, S. Q. T., O. S. Ibrahim & A. A. Khashan. 2024. antibacterial activity and phytochemical analysis of moringa oleifera extract against Staphylococcus aureus identified by routine and molecular methods. Medical Journal of Babylon, 21(3): 724-728.
https://doi.org/10.4103/mjbl.mjbl_1765_23 DOI: https://doi.org/10.4103/MJBL.MJBL_1765_23
AL-Sabagh, F. H., K.K. Ghaima & A.H. Sh AL-Dabbagh. 2023 The antibacterial activity of LL-37 peptide against multidrug-resistant Pseudomonas aeruginosa isolated from burn infections. Revista Bionatura, 8(1): 69. https://doi.org/10.21931/rb2023.08.01.69 DOI: https://doi.org/10.21931/RB/2023.08.01.69
Ali, K. A., & E. Ghassan. 2024. detection of efflux pump MexX and MexY genes in multidrug resistant Pseudomonas aeruginosa. Iraqi Journal of Science, 65(10): 5455-5465.
https://doi.org/10.24996/ijs.2024.65.10.11 DOI: https://doi.org/10.24996/ijs.2024.65.10.11
American Association of Cereal Chemists (AACC). 2001. Method 08-01. The Association St. Paul, M.N.pp:32-33.
Anand, M. & R. Basavaraju. 2021. A review on phytochemistry and pharmacological uses of Tecoma stans (L.) Juss. ex Kunth. Journal of Ethnopharmacology, 265: 113270. https://doi.org/10.1016/j.jep.2020.113270 DOI: https://doi.org/10.1016/j.jep.2020.113270
Avakh, A., D. G. Grant, J. M. Cheesman, T. Kalkundri, & S. Hall. 2023. The Art of War with Pseudomonas aeruginosa: Targeting Mex Efflux Pumps Directly to Strategically Enhance Antipseudomonal Drug Efficacy. Antibiotics (Basel, Switzerland), 12(8): 1304. https://doi.org/10.3390/antibiotics12081304 DOI: https://doi.org/10.3390/antibiotics12081304
Bakr, R. O., A. M. Fayed, A. M. Salem, & S. A. Hussein. 2019. Tecoma stans: alkaloid profile and antimicrobial activity. Journal of Pharmacy and Bioallied Sciences, 11(4):341-347.
https://doi.org/10.4103/jpbs.jpbs_79_19
Balasubramanian, B. S., H. K. Suresha, K. H. Ahalya Devi, H. K. Niveditha, D. Kathirvel, & R. Sharan, 2024. A review on Tecoma Stans Juss plant. International Journal of Pharmacognosy, 11(10): 514-518. https://doi.org/10.13040/IJPSR.0975-8232.IJP.11(10).514-18 DOI: https://doi.org/10.13040/IJPSR.0975-8232.IJP.11(10).514-18
Cary, N. 2012. Statistical Analysis System, User's Guide. Statistical. Version 9. SAS. Inst. Inc. USA. PP:23-25
Dairi, S., A. M. Carbonneau, T. Galeano-Diaz, H. Remini, F. Dahmoune, O. Aoun, et al. 2017. Antioxidant effects of extra virgin olive oil enriched by myrtle phenolic extracts on iron-mediated lipid peroxidation under intestinal conditions model. Food Chem., 237:297–304. https://pubmed.ncbi.nlm.nih.gov/28763999/ DOI: https://doi.org/10.1016/j.foodchem.2017.04.106
Darweesh, A. H. K. & J. K. Luti. 2024. Utilization of Limosilactobacillus fermentum Cells in a Gel Formula as a Dermal Probiotic against MDR Pseudomonas aeruginosa Associated with Burn Wound Infection. Iraqi Journal of Science, 65(12):6970-6985.
https://doi.org/10.24996/ijs.2024.65.12.14. DOI: https://doi.org/10.24996/ijs.2024.65.12.14
Dias, M. C., D. C. Pinto, & A. M. Sliva. 2021. Plant Flavonoids: Chemical Characteristics and Biological Activity. Molecules, 26(17):5377. https://doi.org/10.3390/molecules26175377 DOI: https://doi.org/10.3390/molecules26175377
Durgadevi, T., K. Devika, G. Anburaj, & T. C. Valliammai. 2024. To determine the antioxidant mechanism of action, biochemical compounds in Tecoma stans L. Juss. ex Kunth were analyzed using GC-MS, HPLC, UV-VIS, and FTIR techniques. Ecology, Environment and Conservation (0971765X), 30.
http://doi.org/10.53550/EEC.2024.v30i06s.067 DOI: https://doi.org/10.53550/EEC.2024.v30i06s.067
Gonçalves, T.P.R., Parreira, A.G., dos Santos Zanuncio, V.S., de Souza Farias, K., da Silva, D.B., & dos Santos Lima, L.A.R. (2022). Antibacterial and antioxidant properties of flowers from Tecoma stans (L.) Juss. ex Kunth (Bignoniaceae). South African Journal of Botany, 144: 156–165.
https://doi.org/10.1016/j.sajb.2021.08.028 DOI: https://doi.org/10.1016/j.sajb.2021.08.028
Harbone J. B. 1984. Phytochemical methods. A guide to modern techniques of plant analysis. 2nd ed.; Chapman and Hall. London. pp: 288. https://doi.org/10.1016/0048-4059(85)90073-6 DOI: https://doi.org/10.1016/0048-4059(85)90073-6
Harborne, A. J. 1998. Phytochemical methods a guide to modern techniques of plant analysis. springer science and business media.
Hilal, H. A. (2023). Molecular and epidemiological study of pseudomonas aeruginosa isolated from burn patients in Baghdad. Cardiometry, (29): 116-121.
https://doi.org/10.18137/cardiometry.2023.29.116121 DOI: https://doi.org/10.18137/cardiometry.2023.29.116121
Jaffer, H. J., J. M. Mahmod, M. A. Jawad, A. Naji, & A. AL-Naib. 1988. Phytochemical and biological screening of some Iraqi plants. Fitoterapia, 59: 229-233.
Javid, T., M. Adnan, A. Tariq, B. Akhtar, R. Ullah, & M. N. Abd El Salam. 2015. Antimicrobial activity of three medicinal plants (Artemisia indica, Medicago falcate and Tecoma stans). African Journal of Traditional, Complementary and Alternative Medicines, 12(3): 91-96.
http://dx.doi.org/10.4314/ajtcam.v12i3.11 DOI: https://doi.org/10.4314/ajtcam.v12i3.11
Jayaprakasha, G. K., P. R. Singh, & K. K. Sakariah. 2001. Antioxidant activity of grape seeds (Vitis vinifera). Food Chemistry, 73: 285-290. https://doi.org/10.1016/s03088146(00)00298-3 DOI: https://doi.org/10.1016/S0308-8146(00)00298-3
Jin, J., A. O. Koroleva, T. Gibson, J. Swanston, J. Magan, A. Y. Zhang, et al. 2009. Analysis of phytochemical composition and chemoprotective capacity of rocket (Eruca sativa and Diplotaxis enuifolia) leafy salad following cultivation in different environments. Journal of Agricultural and Food Chemistry, 57(12): 5227-5234. https://doi:10.1021/jf9002973 DOI: https://doi.org/10.1021/jf9002973
Lee, B. 2020. Libo plant potential (Ficus variegata, Blume) as source of potential pharmaceutical materials. International Journal of Science and Society, 2(3): 123-134. https://doi.org/10.54783/ijsoc.v2i3.134 DOI: https://doi.org/10.54783/ijsoc.v2i3.134
Mohsen, Z. A. & F. W. Abas. 2024. Study of the Effectiveness of Nano Pumpkin Peel Extract as an Antioxidant. In IOP Conference Series: Earth and Environmental Science, 1371(10), 102008. IOP Publishing. https://doi.org/10.1088/1755-1315/1371/10/102008 DOI: https://doi.org/10.1088/1755-1315/1371/10/102008
Morita, Y., J. Tomida, & Y. Kawamura. (2014). Responses of Pseudomonas aeruginosa to antimicrobials. Frontiers in Microbiology, 4(32): 422-430. https://doi.org/10.fmicb.2013.00422. DOI: https://doi.org/10.3389/fmicb.2013.00422
Mradu, G., S. Saumyakanti, M. Sohini, & M. Arup. 2012. HPLC profiles of standard phenolic compounds present in medicinal plants. International Journal of Pharmacognosy and Phytochemical Research, 4(3): 162-167.
https://doi:10.7897/2230-8407.041021 DOI: https://doi.org/10.7897/2230-8407.041021
Ngoupayo, J., C. Talla1, K. Y. Dongmo, K. N. Bijou-Lafortune, F. F. Boyom, & M. E. Mpondo. 2024. Phytochemical screening and in vivo anti-inflammatory activities of hydroethanolic extract of Tecoma stans leaves. Scholars Academic Journal of Pharmacy, 13(1): 32–38. https://doi.org/10.36347/sajp.2024.v13i07.004 DOI: https://doi.org/10.36347/sajp.2024.v13i07.004
Ogunmoyole, T., S. Inaboya, O. J. Makun, & J. I. Kade. 2013. Differential antioxidant properties of ethanol and water soluble phytochemicals of false nutmeg (Monodora myristica) seeds. International Journal of Biotechnology and Biochemistry, 2: 253-262. https://doi.org/10.1016/j.gendis.2019.04.001
Pachori, P., R. Gothalwal, & P. Gandhi. 2019. Emergence of antibiotic resistance Pseudomonas aeruginosa in intensive care unit; a critical review. Genes and Diseases, 6(2): 109-119.
https://doi.org/10.1016/j.gendis.2019.04.001 DOI: https://doi.org/10.1016/j.gendis.2019.04.001
Razmavar, S., A. A. Mahmood, I. B. Salmah & H. Pouya. 2014. Antibacterial Activity of Leaf Extracts of Baeckea frutescens against Methicillin-Resistant Staphylococcus aureus. BioMedical Research International, Article ID 521287, pp:5. DOI: https://doi.org/10.1155/2014/521287
https://doi.org /10.1155 /2014 /521287
Sailaja, V., M. M., & V. Neeraja. 2016. Quantitative phytochemical analysis of some medicinal plant seed by using variousorganic solvents." Journal of Pharmacognosy and Phytochemistry 5 (2):30.
Salem, M. Z., M. Y. Gohar, M. L. Camacho, A. N. El-Shanhorey, & Z. A. Salem. 2013. Antioxidant and antibacterial activities of leaves and branches extracts of Tecoma stans (L.) Juss. Ex Kunth against nine species of pathogenic bacteria. African Journal of Microbiology Research, 7(5): 418-426. https://doi.org/10.5897/AJMR12.2274 DOI: https://doi.org/10.5897/AJMR12.2274
Selvan, M. T., M. Ramesh, F. A. Sahayaraj, J. H. Prabu, & J. K. Nagarajan. 2024. Extraction and characterization of novel fibers from Tecoma stans L. bark for use as reinforcement in polymer composites. International Journal of Biological Macromolecules, 270: 132492. https://doi.org/10.1016/j.ijbiomac.2024.132492 DOI: https://doi.org/10.1016/j.ijbiomac.2024.132492
Singh, S., T. C. Miller, P. Singh, R. Sharma, N. Rana, A. K. Dhakad, & K. R. Dubey. 2024. A comprehensive review on ecology, life cycle and use of Tecoma stans (bignoneaceae). Botanical Studies, 65(1): 6. https://doi.org/10.1186/s40529-024-00412-4. DOI: https://doi.org/10.1186/s40529-024-00412-4
Verma, S. (2016). Phytochemical and pharmacological review study on Tecoma stans L. Journal of Medicinal Plants Studies, 4(5): 162-164. https://doi.org/10.4103/jpbs.jpbs_79_19 DOI: https://doi.org/10.4103/jpbs.JPBS_79_19
Wichayapreechar, P., A. Prasansuklab, P. Charoongchit, & R. Charoenjittichai. 2024. The Potential of Tecoma stans (Linn.) Flower Extract as a Natural Antioxidant and Anti-Aging Agent for Skin Care Products. Cosmetics, 11(6): 214. https://doi.org/10.3390/cosmetics11060214 DOI: https://doi.org/10.3390/cosmetics11060214
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