Enhancing nutritional value and antioxidants in blackberry by foliar potassium sulfate and phenylalanine under soilless cultivation

Authors

  • Alaa J.A. Al-Tameemi Department of Horticulture and Landscape Gardening,College of Agricultural Engineering Sciences, University of Baghdad, Baghdad, Iraq.
  • Nazik H. Khalil Department of Horticulture and Landscape Gardening,College of Agricultural Engineering Sciences, University of Baghdad, Baghdad, Iraq.

DOI:

https://doi.org/10.36103/66mmc885

Keywords:

anthosanins, organic media, , total phenols

Abstract

The aim of this study to enhance the nutritional value and antioxidants in the leaves and fruits of blackberry plants by cultivating them using soilless conducted at Research Station of the College of Agricultural Engineering Sciences-University of Baghdad in the 2023–2024 seasons. Three factors included the first factor: cultivation in organic media of local and imported plant waste: The media included licorice root waste (C3) and coconut husk waste (C2), compared to field soil (C1). The second factor was Folior application of potassium sulfate (S) in concentrations (0, 2.5, 5) g.l⁻¹ and the third factor was foliar applicates with amino acid phenylalanine (A) in concentrations (0, 100, 200) mg. l⁻¹ in factorial experiment as a completely randomized design (R.C.B.D) with three replications. The results indicated the superiority of the treatment with licorice residue medium and phenylalanine at a concentration of 200 mg.l⁻¹ with 2.5 g.l⁻¹ of potassium sulfate (C3A2S1) with the highest concentration of total carotene in the leaves (13.33 mg 100g⁻¹) and the highest concentration of phenols, anthocyanin, and DPPH in the fruits (198.26, 534.5 mg100g⁻¹, 89.41%) respectively. The treatment C3A1S2 excelled with the highest concentration of total carotene and folic acid in the fruits (0.81, 2.62 mg 100g⁻¹) respectively.

References

Adams, Z. P., J. Ehlting & R. Edwards. (2019). The regulatory role of shikimate in plant phenylalanine metabolism, Journal of Theoretical Biology, 462:158-170.

DOI: 10.1016/j.jtbi.2018.11.005 DOI: https://doi.org/10.1016/j.jtbi.2018.11.005

Ahmed, A., A. Sattar, H. Hussein, & D. Khurshed (2023). Effect of Humic acid and Potassium Sulfate spraying on growth and yield of Strawberries (Fragaria x ananassa Duch.) Kirkuk University Journal for Agricultural Science (: 3 ) 14 . p 251-256. DOI: 10.58928/KU23.14326 DOI: https://doi.org/10.58928/KU23.14326

Ali, S., A. Hafeez, X. Ma, S. A. Tung; & G. Yang (2020). Relative Potassium ratio balanced the Carbon-Nitrogen assimilation in cotton leaf under reducing Nitrogen application. Journal of Soil Science and Plant Nutrition, 20, 761-774.

DOI: 10.1007/s42729-019-00163-3 DOI: https://doi.org/10.1007/s42729-019-00163-3

Bihari, C., S. Ahamad, M. Kumar, A. Kumar, A. D. Kamboj, S.Singh, V. Srivastava, & P. Gautam. (2023). Innovative soilless culture techniques for horticultural crops:acompre-hensive review. International Journal of Environment and Climate Change.13(10):4071–4084.. DOI: 10.9734/IJECC/2023/v13i103084 DOI: https://doi.org/10.9734/ijecc/2023/v13i103084

Gaurat, J. O., D. Cruz, J. E. Lima, C. G. Sena, N. C. Santos, & E. D. B. Silva( 2020). Foliar nutrient contents and yield performance of blackberry with potassium fertilization. Pesquisa Agropecuária Brasileira 55: e02198. DOI: 10.1590/s1678-3921.pab2020.v55.02198 DOI: https://doi.org/10.1590/s1678-3921.pab2020.v55.02198

Ghorbel, R., J. C., Hatice Malayoğlu, & Numan Çetin(2021). Hydroponics Soilless Farming: the Future of Food and Agriculture–Areview. 1(1): 37–49 DOI: 10.52460/issc.2021.007 DOI: https://doi.org/10.52460/issc.2021.007

Gil-Martínez, L., N. Mut-Salud, J. A. Ruiz-García, A. Falcón-Piñeiro, M. Maijó-Ferré, A. Baños, J. M. Delatorre Ramírez, E. Guillamón, V. Verardo, & A. M. Gómez Caravaca(2023). Phytochemicals determination, and antioxidant, antimicrobial, anti-inflammatory and anticancer activities of blackberry fruits. Foods 12 (7): 1505: 2–18. https://doi.org/10.3390/foods12071505 DOI: https://doi.org/10.3390/foods12071505

Hasanuzzaman, M., M. A. Hossain, J. A. Teixeira da Silva, &M. Fujita(2018). Potassium: A vital regulator of plant responses and tolerance to abiotic stresses. Agronomy.8(3):31. https://doi.org/10.3390/agronomy8030031 DOI: https://doi.org/10.3390/agronomy8030031

Hyun, M. W., Y. H. Yun, J. Y. Kim, & H. K. Seong Hwan (2011). Fungal and plant phenylalanine ammonia-lyase. Mycobiology 39 (4): 257–265. https://doi.org/10.5941/MYCO.2011.39.4.257 DOI: https://doi.org/10.5941/MYCO.2011.39.4.257

Khadam A.A., A. M. Hasan & N. H. Altaee(2020). Effect of Spray with Phenylalanine and Magnesium Sulfate on Growth and Gene expression Level of Myba2 Gene for Black Grapevine Cv. Dase AL-Enz. Int. J. Agricult. Stat. Sci. Vol. 16, Supplement 1, pp. 1689-1700

chrome extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.researchgate.net/profile/Ahmed-Hasan-33/publication/352954219_Effect_of_spray_with_phenylalanine_and_magnesium_sulfate_on_growth_and_gene_expression_level_of_myba2_gene_for_black_grapevine_cv_dase_al-ENZ/links/60e0bce5458515d6fbfa1405/EFFECT-OF-SPRAY-WITH-PHENYLALANINE-AND-MAGNESIUM-SULFATE-ON-GROWTH-AND-GENE-EXPRESSION-LEVEL-OF-MYBA2-GENE-FOR-BLACK-GRAPEVINE-CV-DASE-AL-ENZ.pdf

Kumar, T. V., & R. P. Verma(2024). A comprehensive review on soilless cultivation for sustainable agriculture. Journal of Experimental Agriculture International 46 (6): 193–207. https://doi.org/10.9734/jeai/2024/v46i62470. DOI: https://doi.org/10.9734/jeai/2024/v46i62470

Lee., H., Z. Wang, Z. Deng, & Y. Wang (2024). Assessment of Six Blackberry Cultivars Using a Combination of Metabolomics, Biological Activity, and Network Pharmacology Approaches. Antioxidants, Available from: 10.3390/antiox13030319. DOI: https://doi.org/10.3390/antiox13030319

Manzoor, M. F., A. Hussain, D. Tazeddinova, A. Abylgazinova, & B. Xu( 2022). Assessing the nutritional-value-based therapeutic potentials and non-destructive approaches for mulberry fruit assessment: an overview. Computational Intelligence and Neuroscience 2022: 6531483, 1-16 https://doi.org/10.1155/2022/6531483 . DOI: https://doi.org/10.1155/2022/6531483

Massa, D., J. J. Magán, F. F. Montesano, & N. Tzortzakis (2020). Minimizing water and nutrient losses from soilless cropping in southern Europe. Agricultural Water Management 241: 106395. https://doi.org/10.1016/j.agwat.2020.106395 DOI: https://doi.org/10.1016/j.agwat.2020.106395

Memete, A. R., I. S˘arac, A. C. Teusdea, R. Bud˘au, M. Bei, & S. I. Vicas( 2023). Bioactive compounds and antioxidant capacity of several blackberry (Rubus spp.) fruits cultivars grown in Romania. Horticulturae 9: 556, 1–21. DOI: 10.3390/horticulturae9050556 DOI: https://doi.org/10.3390/horticulturae9050556

Okatan, V. (2020) .Antioxidant properties and phenolic profile of the most widely appreciated cultivated berry species: a comparative study. Folia Horticulturae 32 (1): 79–85.

DOI: https://doi.org/10.2478/fhort-2020-0008 DOI: https://doi.org/10.2478/fhort-2020-0008

Oosalo, A.A., L. Naseri, A. Alirezalu, R. Darvish Zadeh, & S.N. Ebrahimi(2024). Exogenous phenylalanine application effects on phytochemicals, antioxidant activity, HPLC profiling, and PAL and CHS genes expression in table grapes (Vitis vinifera cv. Qzl Ouzum’). BMC Plant Biol 24(1) 1216 https://doi.org/10.1186/s12870-024-05934-4 DOI: https://doi.org/10.1186/s12870-024-05934-4

Ortel, C. T., L. Roberts, &J. C. Rupe (2024). A review of the interaction between potassium nutrition and plant disease control. Agrosystems, Geosciences & Environment 111.

https://doi.org/10.1002/agg2.20489 DOI: https://doi.org/10.1002/agg2.20489

pereira, I. d. S., L. M. A. Goncxalves, G. K. Vignolo, & L. E. C. Antunes (2015). Potassium fertilization affects floricane mineral nutrient content, growth, and yield of blackberry grown in Brazil. HortScience 50 (8): 1234–1240. https://doi.org/10.21273/HORTSCI.50.8.1234 DOI: https://doi.org/10.21273/HORTSCI.50.8.1234

Sardans, J., & J. Penuelas (2021). Potassium control of plant functions: ecological and agricultural implications. Plants 10 (2): 1–31. https://doi.org/10.3390/plants10020419 DOI: https://doi.org/10.3390/plants10020419

Souza, V. P., R. Rithika& R. Malathi(2024). Comparative Analysis of Cocopeat and Soil on Plant Constituents. Journal of Chemical Health Risks www.jchr.org JCHR (2024) 14(3), 2611-2622 .

www.jchr.org. https://doi.org/10.22034/jchr.2024.2061267.1240

Subbiah, V., B. Zhong, M. A. Nawaz, C. J. Barrow, F. R. Dunshea, & H. A. R. Suler(2021). Screening of phenolic compounds in Australian grown berries by LC-ESI-QTOF-MS/MS and determination of their antioxidant potential. Antioxidants 10:126.

https://doi.org/10.3390/antiox10010026 DOI: https://doi.org/10.3390/antiox10010026

Vega, E. N., A. K. Molina, C. Pereira, M. I. Dias, S. A. Heleno, P. Rodrigues, I. P. Fernandes, M. F. Barreiro, D. Stojković, & M. Soković (2021). Anthocyanins from Rubus fruticosus L. and Morus nigra L. applied as food colorants:a natural alternative. Plants 10:1181.

https://doi.org/10.3390/plants10061181 DOI: https://doi.org/10.3390/plants10061181

Yahya, A., A. S. Shaharom, R. Mohamad, & A. Selamat (2009). Chemical and physical characteristics of cocopeat-based media mixtures and their effects on the growth and development of Celosia cristata. American Journal of Agricultural and Biological Sciences 4 (1): 63–71. DOI: 10.3844/AJAB.2009.63.71 DOI: https://doi.org/10.3844/AJAB.2009.63.71

Yoo, H., J. R. Widhalm, Y. Qian, H. Maeda, B. R. Cooper, A. S. Jannasch, I. Gonda, E. Lewinsohn, D. Rhodes, & N. Dudareva (2013). An alternative pathway contributes to phenylalanine biosynthesis in plants via a cytosolic tyrosine:phenylpyruvate amino transferase .Nature Communications 4: Article 2833, 1– DOI: 10.1038/ncomms3833 DOI: https://doi.org/10.1038/ncomms3833

Zia-Ul-Haq, M., M. Riaz, V. de Feo, H. Jaafar, &M. Moga (2014). Rubus fruticosus L.: Constituents, biological activities and health-related uses. Molecules 19: 10998–11029. doi: 10.3390/molecules190810998 DOI: https://doi.org/10.3390/molecules190810998

Downloads

Published

2026-08-31

Issue

Section

Articles

How to Cite

Al-Tameemi , A., & Khalil , N. (2026). Enhancing nutritional value and antioxidants in blackberry by foliar potassium sulfate and phenylalanine under soilless cultivation. IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 57(8), 2133-2147. https://doi.org/10.36103/66mmc885