EFFECT OF ADDING DIETARY ORGANIC TRACE MINERALS (ZN, CR, AND MN) AND THEIR MIXTURES ON PHYSIOLOGICAL TRAITS LAYING HENS
DOI:
https://doi.org/10.36103/rjjqjm32Keywords:
blood, bones, layer chickens, Lohmann, mineralsAbstract
ABSTRACT
This study was conducted to investigate the effect of adding dietary organic trace minerals (zinc, chromium, and manganese) and their mixtures on the levels of zinc, manganese, chromium, calcium, and phosphorus on some physiological traits. The experiment included the following treatments: Treatment (T1) served as control group without any addition. Treatment (T2) involved the addition of zinc (40 mg/kg diet). treatment (T3) included the addition of chromium (0.4 mg/kg diet). treatment (T4) consisted of the addition of manganese at a concentration of 40 mg/kg diet. Treatment (T5) involved a combination of zinc (40 mg), manganese (40 mg), and chromium (0.4 mg) per kg diet. The results demonstrated significant improvements in most of the studied traits. In terms of blood serum, there was a significant (p < 0.05) increase in the levels of zinc, manganese, and calcium in all treatments containing mineral additives and their mixture compared to control group, which showed a noticeable decline. However, non. significant differences were observed in the serum levels of chromium and phosphorus across the treatments. Regarding bone mineral levels, there was a significant increase (p < 0.05) in zinc, chromium, manganese, phosphorus, and calcium in all supplemented treatments compared to the control group. These findings suggest that the addition of organic trace minerals, either individually or as a mixture, enhances the physiological levels of these minerals in both blood serum and bones, thereby improving mineral bioavailability.
Received: 22/11/2024
Accepted: 9/2/2025
Published: 31/7/2026
References
REFERENCES
Abdallah, A. G., O. M. El-Hoseiny & Abdel-Latif. K. O. 2009. Influence of some dietary organic mineral supplementations. Int. J. Poult. Sci., 8(3): 291–298. DOI: http://dx.doi.org/10.3923/ijps.2009.291.298 . DOI: https://doi.org/10.3923/ijps.2009.291.298
Alagawany, M., Elmers, S. S., Farag, M. R., Tiwari, R. Yatoo, M. I. Karthik, K., Michalak I. & Dhama. K. 2021. Nutritional significance of amino acids, vitamins and minerals as nutraceuticals in poultry production and health–a comprehensive review. Veterinary Quarterly, 41(1), 1–29. https://doi.org/10.1080/01652176.2020.1857887 DOI: https://doi.org/10.1080/01652176.2020.1857887
Amata, I. A., 2013. Chromium in livestock nutrition: A review. Global Advanced Research Journal of Agricultural Science, 2(12), 289–306.
Azad, S. K., Shariatmadari, F. M. K., Torshizi, & Ahmadi, H.. 2017. Effect of zinc concentration and source on performance, tissue mineral status, activity of superoxide dismutase enzyme and lipid peroxidation of meat in broiler chickens. Animal Production Science, 58(10:1837-1846. DOI: https://doi.org/10.1071/AN15758
Belloir, P., Lessire, M. Lambert, W., Corrent,E. Berri, C. & Tesseraud, S. 2019. Changes in body composition and meat quality in response to dietary amino acid provision in finishing broilers. Animal, 13(5): 1094–1102. https://doi.org/10.1017/S1751731118002306 DOI: https://doi.org/10.1017/S1751731118002306
Burezq, H. A. 2021. Utilization of eggshells as valuable products for sustainable ecosystem and agriculture. Poultry Science Journal, 9(2), 147–165. doi: 10.22069/psj.2021.19210.1699
Chen, X., Yang, G. Zhang, B. Li, F. Liu, L. & Li, F. 2020. Effects of manganese-supplemented diets on growth performance, blood biochemistry, nitrogen metabolism and skeletal development of rex rabbits." Journal of Trace Elements in Medicine and Biology, 61, 126543. doi: 10.1016/j.jtemb.2020.126543 DOI: https://doi.org/10.1016/j.jtemb.2020.126543
Duncan's, B. D., 1955. Multiple Range and Multiple F-test. Biometrics, 11: 1-42. DOI: https://doi.org/10.2307/3001478
Elgeddawy, S. A., Shaheen H. M., El-Sayed Y. S., Elaziz M. Abd, Darwish A., Samak D., Batiha G. E., Mady R. A., Bin-Jumah M., A. A. & Alagawany, M., 2020. Effects of the Allam, dietary inclusion of a probiotic or prebiotic on florfenicol pharmacokinetic profile in broiler chicken. Journal of Animal Physiology and Animal Nutrition, 104(2), 549–557. doi: 10.3390/ani10030452 DOI: https://doi.org/10.1111/jpn.13317
Fakharzadeh, S., Hafizi M., Baghaei M. A., Etesami M., Khayamzadeh M., Kalanaky S., Akbari, M. E., & . Nazaran, M. H, 2020. Using nanochelating technology for biofortification and yield increase in rice. Scientific Reports, 10(1), 4351. doi: 10.1038/s41598-020-60189-x DOI: https://doi.org/10.1038/s41598-020-60189-x
FAO. 2020. Food Outlook. Food and Agricultural organization .United State. http://www.fao.org/3/ca9509en/ca9509en.pdf
Ford, M. J. 2009. Effects of feeding different forms of zinc and copper on the performance and tissue mineral content of chicks. Poultry Science, 88(10), 2171–2175. doi: 10.3382/ps.2009-00117 DOI: https://doi.org/10.3382/ps.2009-00117
Gálvez, F., Domínguez R., Maggiolino,A. Pateiro M., Carballo, J. De Palo P., Barba F. J., & Lorenzo J. M., 2020. Meat Quality of commercial chickens reared in different production systems: industrial, range and organic. Annals of Animal Science, 20(1), 263–285. doi: 10.2478/aoas-2019-0067 DOI: https://doi.org/10.2478/aoas-2019-0067
Ghoreyshi, S. M., Omri B., Chalghoumi R., Bouyeh M., Seidavi A., Dadashbeiki M., Lucarini M., Durazzo A., van den Hoven R., & Santini A., 2019. Effects of dietary supplementation of L-carnitine and excess lysine-methionine on growth performance, carcass characteristics, and immunity markers of broiler chicken. Animals, 9(6), 362. doi: 10.3390/ani9060362 DOI: https://doi.org/10.3390/ani9060362
Haq, Z., Jain R. K., Khan N., Dar,M. Y. Ali S., Gupta M., & Varun T. K., 2016. Recent advances in role of chromium and its antioxidant combinations in poultry nutrition: A review. Veterinary World, 9(12), 1392. doi 10.14202/vetworld.2016.1392-1399 DOI: https://doi.org/10.14202/vetworld.2016.1392-1399
Horváth, M., & Babinszky L., 2018. Impact of selected antioxidant vitamins (Vitamin A, E and C) and micro minerals (Zn, Se) on the antioxidant status and performance under high environmental temperature in poultry. A review. Acta Agriculturae Scandinavica, Section A—Animal Science, 68(3), 152–160. doi: 10.1080/09064702.2019.1611913 DOI: https://doi.org/10.1080/09064702.2019.1611913
Idowu, O. M. O., Ajuwon R. O., Oso A. O., & Akinloye O. A., 2011. Effects of zinc supplementation on laying performance, serum chemistry and Zn residue in tibia bone, liver, excreta and eggshell of laying hens. International Journal of Poultry Science, 10(3), 225–230. doi: 10.1007/s12011-024-04121-8 DOI: https://doi.org/10.3923/ijps.2011.225.230
Karam, M., Soliman N. K., N. El-Medany M., & El-Wardany I., 2007. Effect of supplemental chromium on performance, blood constituents and digestive enzymes activity of broiler chicks. Journal of Animal and Poultry Production, 32(9), 7199–7209. DOI: https://doi.org/10.21608/jappmu.2007.220589
Khatun, A., S. D. Chowdhury, B. Roy C., Dey B., Haque A., & Chandran B., 2019. Comparative effects of inorganic and three forms of organic trace minerals on growth performance, carcass traits, immunity, and profitability of broilers. Journal of Advanced Veterinary and Animal Research, 6(1), 66–73.doi: 10.5455/javar.2019.f313 DOI: https://doi.org/10.5455/javar.2019.f313
Kong, J., Qiu T., Yan X., Wang L., Chen Z., Xiao G., Feng X., & Zhang H., 2022. Effect of replacing inorganic minerals with small peptide chelated minerals on production performance, some biochemical parameters and antioxidant status in broiler chickens. Frontiers in Physiology, 13, 1027834. doi: 10.3389/fphys.2022.1027834 DOI: https://doi.org/10.3389/fphys.2022.1027834
Kwiecień, M., Winiarska-Mieczan A., Milczarek A., Tomaszewska E., & Matras J., 2016. Effects of zinc glycine chelate on growth performance, carcass characteristics, bone quality, and mineral content in bone of broiler chicken. Livestock Science, 191, 43–50. DOI: 10.1016/j.livsci.2016.07.002 DOI: https://doi.org/10.1016/j.livsci.2016.07.005
Lilburn, M. S., & McIntyre, D. R., 2024. An Historical Overview of Zinc in Poultry Nutrition. Poultry Science, 104294. doi: 10.1126/science.127.3303.875 · DOI: https://doi.org/10.1016/j.psj.2024.104294
Malik, M., Hussain S., Malik F., Sultan T., Ali H., Hameed A., Abbasi N., & Usmanghani K., 2011. The effect of supplemental dietary chromium on blood glucose, body weight and liver enzymes of rabbits. Journal of Medicinal Plants Research, 5(16), 3940–3945. doi: 10.5897/JMPR11.740
Niknia, A. D., Vakili R., & Tahmasbi A. M., 2023. Role of zinc-methionine chelates on bone health and eggshell quality in late–phase laying hens. All Life, 16(1), 2162609. DOI: https://doi.org/10.1080/26895293.2022.2162609
doi: 10.1080/26895293.2023.2162609
SAS, Statistical Analysis System, 2001. User's Guide Version 8.2, Cary NC
Sheoran, V. 2017. Organic minerals in poultry. Advances in Research, 12(3), 1–10. doi: 10.9734/AIR/2017/33794 DOI: https://doi.org/10.9734/AIR/2017/37878
Sienkiewicz, S., Arczewska-Włosek A., & Józefiak D., 2014. The efficacy of organic minerals in poultry nutrition: review and implications of recent studies. World's Poultry Science Journal, 70(3), 475–486. doi: 10.1017/S0043933914000514 DOI: https://doi.org/10.1017/S0043933914000531
Sirri, F., Maiorano G., Tavaniello S., Chen J., Petracci M., & Meluzzi A., 2016. Effect of different levels of dietary zinc, manganese, and copper from organic or inorganic sources on performance, bacterial chondronecrosis, intramuscular collagen characteristics, and occurrence of meat quality defects of broiler chickens. Poultry Science, 95(8), 1813–1824. doi: 10.3382/ps/pew064 DOI: https://doi.org/10.3382/ps/pew064
Sunder, G. S., Panda A. K., Gopinath N. C. S., Rao S. R., Raju M. V. L. N., Reddy M. R., & Kumar C. V., 2008. Effects of higher levels of zinc supplementation on performance, mineral availability, and immune competence in broiler chickens. Journal of Applied Poultry Research, 17(1), 79–86. doi: https://doi.org/10.3390/ani909063 DOI: https://doi.org/10.3382/japr.2007-00029
Turek, A., Wieczorek K., & Wolf W. M., 2019. Digestion procedure and determination of heavy metals in sewage sludge—An analytical problem. Sustainability, 11(6), 1753. doi: 10.1093/ps/85.2.273 DOI: https://doi.org/10.3390/su11061753
Xu, C.L., Ji C., Ma Q., Hao K., Jin Z. Y., & Li K., 2006. Effects of a dried Bacillus subtilis culture on egg quality. Poultry Science, 85(2), 273–277. DOI: https://doi.org/10.1093/ps/85.2.364
Yenice, E., Mızrak C., Gültekin M., Atik Z., & Tunca M., 2015. Effects of organic and inorganic forms of manganese, zinc, copper, and chromium on bioavailability of these minerals and calcium in late-phase laying hens. Biological Trace Element Research, 167, 300–307. doi:10.1007/s12011-015-0301-x DOI: https://doi.org/10.1007/s12011-015-0313-8
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Saja A., R. Al-Mahdawi , A. Rahman

This work is licensed under a Creative Commons Attribution 4.0 International License.

2.jpg)
