PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF EUCALYPTUS LEAF ESSENTIAL OIL
DOI:
https://doi.org/10.36103/1djzhp39Keywords:
DPPH assay, Eucalyptus camaldulensis, GC-MS analysis, Medicinal plants, Phytochemical constituentsAbstract
The genus Eucalyptus belongs to the Myrtaceae family and is a large tree with several uses due to its content of some useful active components. These phytochemical components have been used for many years to treat various illnesses. The extraction process and identification of these components are essential points to maximize and develop the level of content. The extraction of essential oil from the eucalyptus leaf was investigated using hydrodistillation based on four locations in the Sulaimani region. Furthermore, the antioxidant activity was also studied using a free radical scavenging assay. The highest essential oil content was noticed in Qara Dagh in both fresh and dry leaves (2.33% and 4.67%), while the lowest level of fresh leaves was detected in Sharbazher (1.27%) and dry leaves in Kalar (2.40%). Fifty-eight chemical components were identified in eucalyptus essential oil using GC-MS analysis. Eucalyptol and α-pinene were found to be major components at all locations. The greatest amounts of eucalyptol and α-pinene were observed in Qara Dagh (52.309±0.967% at 6.163 min) and (14.652±0.194 % at 3.703 min), respectively. The essential oil scavenging effect indicated that the Sharbazher location gave a maximum scavenging effect of 60.156%. Compared to the lowest scavenging effect, which was achieved by the Qara Dagh location with a value of 47.613%.
Received: 16/8/2024
Accepted: 24/11/2024
Published: 30/6/2026
References
Abbaci, H., E. H., Nabti, A. M., Al-Bekairi, S. A., Hagras, M. M., Salem-Bekhit, A., Adjaoud, H. A., Alzahrani, L., Bensidhoum, R., Alenazy, A., Piras, & D., Falconieri, (2023). Comparative bioactivity evaluation of chemically characterized essential oils obtained from different aerial parts of Eucalyptus gunnii Hook. f. (Myrtaceae). Molecules, 28(6): 2638.
https://doi.org/ /10.3390/molecules28062638
Abdulrahman, M. D., (2023). Plants biodiversity utilisation in Bardarash, Kurdistan Region, Iraq. In IOP Conference Series: Earth and Environmental Science, 1185(1): 012034. IOP Publishing. https://doi.org/ 10.1088/1755-1315/1185/1/012034
Abed, K. M., B. M., Kurji, & B. A. Abdul-Majeed, (2015). Extraction and modeling of oil from Eucalyptus camadulensis by organic solvent. Journal of Materials Science and Chemical Engineering, 3: 35-42. https://doi.org/ 10.4236/msce.2015.38006
Ahmed, H. M., (2017). Traditional uses of Kurdish medicinal plant Pistacia atlantica subsp. kurdica Zohary in Ranya, Southern Kurdistan. Genet Resour Crop Evol, 64: 1473–1484.
https://doi.org/ 10.1007/s10722-017-0522-4
Almas, I., E. Innocent, F. Machumi, & W. Kisinza (2021). Chemical composition of essential oils from Eucalyptus globulus and Eucalyptus maculata grown in Tanzania. Scientific African, 12, e00758.
https://doi.org/ 10.1016/j.sciaf.2021.e00758
Anil P, Manish S, R. S. Garvendra, B. Vijay & K. Tarachand (2010). In vitro antioxidant studies of Lagerstroemia speciosa leaves. Pharmacog Journal, 2(10): 357-360. https://doi.org/ 1
0.1016/S0975-3575(10)80109-9
Arun, S., & K. Inderjeet, (2022). Extraction Fuel During Hydro-distillation Modifies Eucalyptus Essential Oil Yield, Phytochemical Quality, and Capital Cost. Current Indian Science, 1(1): e231222212138. https://doi.org/ 10.2174/2210299x01666221223091643
Bachheti, R. K., (2015). Chemical composition and antibacterial activity of the essential oil from the leaves of Eucalyptus globulus collected from Haramaya University, Ethiopia. Der Pharma Chemica. 7 (2): 209-214.
Ben Marzoug, H. N., J., Bouajila, M. Ennajar, A. Lebrihi, F. Mathieu, F. Couderc, M. Abderraba, M. & Romdhane, (2010). Eucalyptus (gracilis, oleosa, salubris, and salmonophloia) essential oils: Their chemical composition and antioxidant and antimicrobial activities. Journal of medicinal food, 13(4): 1005-1012.
https://doi.org/ 10.1089/jmf.2009.0153
Bourakna, Z., Righi, K., & Assia Righi, F. (2022). GC/MS Analysis of Eucalyptus globulus L. (Myrtaceae) leaves essential oil from Algeria and their insecticidal activity against adults of Bactrocera oleae (Rossi)(Diptera; Tephritidae). Journal of Essential Oil-Bearing Plants, 25(4), 876-887. https://doi.org/ 10.1080/0972060X.2022.2129459.
Brooker, M. I. H. (2000). A new classification of the genus Eucalyptus L'Her. (Myrtaceae). Australian systematic botany, 13(1), 79-148.
https://doi.org/ 10.1071/SB98008
Camas, N., Radusiene, J., Ivanuskas, L., Jakstas, V., & Cirak C., (2014). Altitudinal changes in the content of bioactive substances in Hypericum orientale and Hypericum pallens. Acta Physiol Plant, (36): 675-686. https://doi.org/ 10.1007/s11738-013-1446-z
Ciesla, L. M., Wojtunik‐Kulesza, K. A., Oniszczuk, A., & Waksmundzka‐Hajnos, M. (2016). Antioxidant synergism and antagonism between selected monoterpenes using the 2, 2‐diphenyl‐1‐picrylhydrazyl method. Flavour and Fragrance Journal, 31(6), 412-419.
https://doi.org/ 10.1002/ffj.3330.
Čmiková, N., Galovičová, L., Schwarzová, M., Vukic, M. D., Vukovic, N. L., Kowalczewski, P. Ł., & Kačániová, M. (2023). Chemical composition and biological activities of Eucalyptus globulus essential oil. Plants, 12(5), 1076.
https://doi.org/ 10.3390/plants12051076
Cristina, D., P. Malaspina, L. Cornara, A. Smeriglio, D. Trombetta, V. DeFeo, and S. Vanin (2023). Eucalyptus essential oils in pest control: A review of chemical composition and applications against insects and mites. Crop Protection, 176.
https://doi.org/ 10.1016/j.cropro.2023.106319
Dalal, P. 2019. Hydro distillation method extraction of Eucalyptus oil & Lemongrass oil. Social Science Journal, 4, 36-44.
Dhakad, A. K., Pandey, V. V., Beg, S., Rawat, J. M., & Singh, A. (2018). Biological, medicinal, and toxicological significance of Eucalyptus leaf essential oil: a review. Journal of the Science of Food and Agriculture, 98(3), 833-848.
https://doi.org/ 10.1002/jsfa.8600
Dudareva, N., Pichersky, E., & Gershenzon, J. (2004). Biochemistry of plant volatiles. Plant physiology, 135(4), 1893-1902.
https://doi.org/10.1104/pp.104.049981.
Galan, D. M., Ezeudu, N. E., Garcia, J., Geronimo, C. A., Berry, N. M., & Malcolm, B. J. (2020). Eucalyptol (1, 8-cineole): an underutilized ally in respiratory disorders?. Journal of essential oil research, 32(2), 103-110.
https://doi.org/ 10.1080/10412905.2020.1716867
González-Hernández, R. A., Valdez-Cruz, N. A., & Trujillo-Roldán, M. A. (2024). Factors that influence the extraction methods of terpenes from natural sources. Chemical Papers, 78(5), 2783-2810.
https://doi.org/ 10.1007/s11696-024-03339-z
Heikal, A. A. E. M. (2017). Variation in the essential oil content and its composition in Eucalyptus cinerea leaves and its relation to some environmental factors. Journal of Essential Oil-Bearing Plants, 20(4), 995-1005. https://doi.org/ 10.1080/0972060X.2017.1351896
Immaroh, N. Z., Kuliahsari, D. E., & Nugraheni, S. D. (2021, April). Eucalyptus globulus essential oil extraction method. In IOP conference series: earth and environmental science (Vol. 733, No. 1, p. 012103). IOP Publishing.
https://doi.org/ 10.1088/1755-1315/733/1/012103
James, O., Nnacheta, O. P., Wara, H. S., & Aliyu, U. R. (2009). Invitro and invivo studies on the antioxidative activities, membrane stabilization, and cytotoxicity of water spinach (Ipomoea aquatica forsk) from Ibaji ponds, Nigeria. Int. J. PharmTech Res, 1(3), 474-482.
Khalid, K. A., Essa, E. F., Ismaiel, H. M., & Elsayed, A. A. (2020). Effects of geographical locations on essential oil composition of navel orange leaves and flowers. Journal of Essential Oil-Bearing Plants, 23(1), 139-148. https://doi.org/ 10.1080/0972060X.2020.1727369
Kumar, P., Mishra, A. K., Chaudhari, S. K., Sharma, D. K., Rai, A. K., Singh, K., & Singh, R. (2021). Carbon sequestration and soil carbon build-up under Eucalyptus plantation in semi-arid regions of North-West India. Journal of Sustainable Forestry, 40(4), 319-331.
https://doi.org/ 10.1080/10549811.2020.1749856
Labate, C. A., de Assis, T. F., Oda, S., de Mello, E. J., González, E. R., Zauza, E. A. V., & Salvatierra, G. R. (2009). Eucalyptus. Compendium of transgenic crop plants, 35-108.
Maciel, M. V., Morais, S. M., Bevilaqua, C. M. L., Silva, R. A., Barros, R. S., Sousa, R. N., & Souza-Neto, M. A. (2010). Chemical composition of Eucalyptus spp. essential oils and their insecticidal effects on Lutzomyia longipalpis. Veterinary parasitology, 167(1), 1-7.
https://doi.org/ 10.1016/j.vetpar.2009.09.053
Mahmoud, A. R. K., & Muhammad, K. A. A. (2000). Design and analysis of agricultural experiments. University of Mosul-Ministry of Higher Education and Scientific Research, Dar Al Kutub for Printing and Publishing/Iraq.
Mishra, A. K., Sahu, N., Mishra, A., Ghosh, A. K., Jha, S., & Chattopadhyay, P. (2010). Phytochemical screening and antioxidant activity of essential oil of Eucalyptus leaf. Pharmacognosy journal, 2(16), 25-28.
https://doi.org/ 10.1016/S0975-3575(10)80045-8.
Mulyaningsih, S., Sporer, F., Reichling, J., & Wink, M. (2011). Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Pharmaceutical biology, 49(9), 893-899.
https://doi.org/ 10.3109/13880209.2011.553625
Nagappan, D., Sivaraj, M., Periyasamy, S., Kamalanathan, A., Gnanavel, B., & Chinnappan, B. (2024). Phytochemical Analysis and Evaluation of Antifungal Properties of Eucalyptus Oil against Candida Species. Shanlax Int. J. Arts Sci. Humanit, 12, 1-15.
https://doi.org/ 10.34293/ sijash.v12i1.7699
Nataraj, S., Subramanian, M., & Geethalakshmi, V. (2022). Allelopathic effect of Eucalyptus tereticornis smith aqueous leaf extract on Echinochloa crus-galli (L.) P. Beauv. International Journal of Ecology and Environmental Sciences, 2(4).
Oliveira, F. N., Fortes, G. A., Paula, J. R., Ferri, P. H., & Santos, S. C. (2014). Seasonal influence on the essential oil of Eucalyptus microcorys. Natural product communica-tions, 9(4), 1934578X1400900439. https://doi.org/ 10.1177/1934578X14009004
Pant, P., Pandey, S., & Dall'Acqua, S. (2021). The influence of environmental conditions on secondary metabolites in medicinal plants: A literature review. Chemistry & biodiversi-ty, 18(11), e2100345.
https://doi.org/ 10.1002/cbdv.202100345
Pries, R., Jeschke, S., Leichtle, A., & Bruchhage, K. L. (2023). Modes of action of 1, 8-cineol in infections and inflammation. Metabolites, 13(6), 751. https://doi.org/10.3390/metabo13060751
Quan, V., Chalmers, A. C., Jyoti Bhuyan, D., Bowyer, M. C., & Scarlett, C. J. (2015). Botanical, phytochemical, and anticancer properties of the Eucalyptus species. Chemistry & biodiversity, 12(6), 907-924.
https://doi.org/ 10.1002/CHIN.201534276
Sebei, K., Sakouhi, F., Herchi, W., Khouja, M. L., & Boukhchina, S. (2015). Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves. Biological research, 48(1), 7. https://doi.org/ 10.1186/0717-6287-48-7.
Serçe, A., Toptancı, B. Ç., Tanrıkut, S. E., Altaş, S., Kızıl, G., Kızıl, S., & Kızıl, M. (2016). Assessment of the antioxidant activity of Silybum marianum seed extract and its protective effect against DNA oxidation, protein damage and lipid peroxidation. Food technology and biotechnology, 54(4), 455-461. https://doi.org/10.17113/ftb.54.04.16.4323
Surbhi, Kumar, A., Singh, S., Kumari, P., & Rasane, P. (2023). Eucalyptus: phytochemical composition, extraction methods and food and medicinal applications. Advances in Traditional Medicine, 23(2), 369-380. https://doi.org/ 10.1007/s13596-021-00582-7
Tum, P. K., Kasha, G. M., Kithure, J. G., & Mwazighe, F. M. (2016). Optimization of essential oil extraction from Eucalyptus grandis leaves by Clevenger distillation. Journal kenya chemical society, 9(1), 91-102.
Vecchio, M. G., Loganes, C., & Minto, C. (2016). Beneficial and healthy properties of Eucalyptus plants: A great potential use. The Open Agriculture Journal, 10(1). https://doi.org/ 10.2174/1874331501610010052
Yost, J. M., Wise, S. L., Love, N. L., Steane, D. A., Jones, R. C., Ritter, M. K., & Potts, B. M. (2021). Origins, diversity, and naturalization of Eucalyptus globulus (Myrtaceae) in California. Forests, 12(8), 1129.
https://doi.org/ 10.3390/f12081129.
Zhou, L., Li, J., Kong, Q., Luo, S., Wang, J., Feng, S., & Ding, C. (2021). Chemical composition, antioxidant, antimicrobial, and phytotoxic potential of Eucalyptus grandis× E. urophylla leaves essential oils. Molecules, 26(5), 1450.
https://doi.org/ 10.3390/molecules26051450
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