ISOLATION AND IDENTIFICATION OF NEW HALOTOLERANT BACTERIAL STRAINS FROM SALTY SOIL
DOI:
https://doi.org/10.36103/npk28a23Keywords:
antibiotic sensitivity, halophilic bacteria, high salinity, molecular diagnosisAbstract
This study was aimed to isolate a halotolerant bacterial strains, four samples were collected from four sources (salty soil) during February 2023. Five bacterial isolates were obtained using Modified Nutrient Agar (MNA) by raising the concentration of sodium chloride to 5% as a medium for isolation. Bacterial isolates were initially diagnosed based on culture and morphological characteristics. The obtained isolates showed varying sensitivity to five types of antibiotics, including ampicillin, azithromycin, amoxicillin, cefexin, and gentamycin. The isolates also showed varying ability to grow in a temperature range from 10 to 50 ℃. The results indicated that the isolates were able to grow differently in different concentrations of sodium chloride, which included (5, 10, 15, 20, 25 and 30%), two isolates showed their ability to grow at a concentration of 30% of sodium chloride. These two isolates were subjected to molecular diagnosis based on the sequences of the 16s rRNA gene, and the result showed that they are two new, previously undiagnosed strains that were registered at the National Center for Biotechnology Information (NCBI), which are Salinicoccus sp. strain Salman and Oceanobacillus sp. Mostafa.
Received: 15/8/2023
Accepted: 20/12/2023
Published: 30/5/2026
References
Adhvaryu, S., Kiskova, J., Piknova, M., Malinicova, L., Beck, T., Buchtikova, I., & Pristas, P. (2025). The characterization of halophilic polyhydroxyalkanoate-producing bacteria from brine in Solivar near Prešov (Slovakia). World Journal of Microbiology and Biotechnology, 41(12), 505. https://doi.org/10.13005/bbra/1874
Al Zamzami, I. M., Yona, D., Faqih, A. R., & Kurniawan, A. (2025). Halophilic bacteria in biotechnology: A seven-decade scientometric analysis of global research trends, knowledge gaps, and emerging applications (1955–2024). Journal of Ecological Engineering, 26(10), 252-271
doi.org/10.12911/22998993/205826
Andharia, K., Katariya, D., & Kothari, R. (2025). Isolation and Characterization of Several Halophilic Bacteria from Marine Environments. Current Biotechnology.
doi:10.2174/0122115501425807251017044247
Arayes, M. A., Mabrouk, M. E., Sabry, S. A., & Abdella, B. (2021). Diversity and characterization of culturable haloalkaliphilic bacteria from two distinct hypersaline lakes in northern Egypt. Biologia, 76(2), 751-761. https://doi.org/10.1007/s00792-016-0879-x
Chawla, M., Verma, J., Gupta, R., & Das, B. (2022). Antibiotic potentiators against multidrug-resistant bacteria: discovery, development, and clinical relevance. Frontiers in microbiology, 13, 887251. /doi.org/10.3389/fmicb.2022.88725
Fierer, N.; J. A. Jackson; R. Vilgalys; and R. B. Jackson. (2005). Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Applied and Environmental Microbiology. 71(7): 4117-4120.
doi: 10.1128/AEM.71.7.4117-4120.2005
Fitri, D. A., Asih, E. N. N., Kartika, A. G. D., Agustina, N., Fadholi, B., Dewi, K., & Efendy, M. (2022). Morphological characteristics of halophilic bacteria in traditional salt production. Journal of Marine Resources and Coastal Management, 3(1), 1-7. doi: https://doi.org/10.29080/mrcm.v3i01.1360
Kapadia, C., Patel, N., Rana, A., Vaidya, H., Alfarraj, S., Ansari, M. J., & Sayyed, R. Z. (2022). Evaluation of plant growth-promoting and salinity ameliorating potential of halophilic bacteria isolated from saline soil. Frontiers in plant science, 13, doi.org/10.3389/fpls.2022.946217
Matuschek, Erika, FJ. D. Brown, and Gunnar Kahlmeter. (2014). "Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories." Clinical microbiology and infection 20(4): 255-266.
doi:10.1111/1469-0691.12373.
Mesa-Marín, J., E. Mateos-Naranjo, I. D. Rodríguez-Llorente, E. Pajuelo, & S. Redondo-Gómez, (2019). Synergic effects of rhizobacteria increasing use of halophytes in a changing world Halophytes and climate change: adaptive mechanisms and potential uses. Wallingford UK: 240-254. doi:10.1079/9781786394330.0240.
Neagu, S., & Stancu, M. M. (2025). Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production. BioTech, 14(2), 49. doi.org/10.3390/biotech14020049
Olaleye, A. C., Oyewusi, H. A., Akinyede, K. A., Oladipo, O. O., & Oyeyemi, B. F. (2025). Bacterial community structure and secondary metabolite insights from halophiles at Oniru Beach, Lagos. Archives of Microbiology, 207(11), 299. doi.org/10.1007/s10661-020-08888-5
Oren, A. (2010). Industrial and environmental applications of halophilic microorganisms. Environmental technology, 31(8-9): 825-834 doi: 10.1080/09593330903370026
Perez, M. F., Saona, L. A., Farías, M. E., Poehlein, A., Meinhardt, F., Daniel, R., & Dib, J. R. (2021). Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina. Scientific Reports, 11(1), 21459. doi.org/10.1038/s41598-021-00753-1
Puspaningrum, T. C., & H.S. Titah, (2020). The removal of salinity in a reed bed system using mangroves and bacteria in a continuous flow series reactor. Journal of Ecological Engineering, 21(6): 212-223. doi.org/10.12911/22998993/124075.
Saibi, A. N. E., Nas, F., Arab, M., Aissaoui, N., Boukeroui, Y., & Klouche-Khelil, N. (2022). Antimicrobial and enzymatic profiling of halophilic and halotolerant bacteria from a hypersaline lake ‘The Great Sebkha of Oran, Northwestern Algeria’. Geomicrobiology Journal, 39(9), 816-831. doi.org/10.1080/01490451.2022.2079777.
Shinde, V. D., & R. S. Thombre, (2016). Antibiotic resistance profiling of marine halophilic bacteria and haloarchaea. Journal of Applied Pharmaceutical Science, 6(10):132-137. doi: 10.7324/JAPS.2016.601018
Teles, E. A. P., Xavier, J. F., Arcênio, F. S., Amaya, R. L., Gonçalves, J. V. S., Rouws, L. F. M., & Coelho, I. S. (2024). Characterization and evaluation of potential halotolerant phosphate solubilizing bacteria from Salicornia fruticosa rhizosphere. Frontiers in Plant Science, 14, 1324056. doi.org/10.3389/fpls.2023.1324056
Thompson, T. P., & Gilmore, B. F. (2024). Exploring halophilic environments as a source of new antibiotics. Critical reviews in Microbiology, 50(3), 341-370. doi.org/10.1080/1040841X.2023.2197491
Tourova, T. P., Sokolova, D. S., Semenova, E. M., Ershov, A. P., Grouzdev, D. S., & Nazina, T. N. (2022). Genomic and physiological characterization of halophilic bacteria of the genera Halomonas and Marinobacter from petroleum reservoirs. Microbiology, 91(3), 235-248. doi.org/10.1007/s00792-010-0312-9
Vreeland, R. H. (2020). Taxonomy of halophilic bacteria. In The Biology of Halophilic Bacteria: 105-134. CRC Press doi: 10.1201/9781003069140.
Yadav, D., A. Singh, N. Mathur, A. Agarwal, & J. Sharma, (.2021). Isolation of halophilic bacteria and their screening for extracellular enzyme production. Journal of Scientific & Industrial Research, 80(7): 617-622.
doi: 10.56042/jsir.v80i7.39611
Yoo, Y., Lee, H., Lee, J., Khim, J. S., & Kim, J. J. (2023). Insights into saline adaptation strategies through a novel halophilic bacterium isolated from solar saltern of Yellow sea. Frontiers in Marine Science, 10, 1229444. doi.org/10.3389/fmars.2023.1229444
Youn, H. Y., & Seo, K. H. (2022). Isolation and characterization of halophilic Kocuria salsicia strains from cheese brine. Food science of animal resources, 42(2), 252. doi: 10.5851/kosfa.2022.e1
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Copyright (c) 2026 Ahmed Shihab Salman, Elham Ismael AL-Shamary
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